• We studied four independent hESC lines HES H

  2018-10-22

  

  We studied four independent hESC lines: HES3, H1, ESI-035, and Shef5. Cultures from these four cell lines that had been reported to contain a 20q11.21 CNV were paired with cultures from previous passages that did not appear to be positive for the CNV by SNP array (Amps et al., 2011). Hereafter, we r

• br Discussion Xenotransplantation of human and nonhuman prim

  2018-10-22

  

  Discussion Xenotransplantation of human and nonhuman primate adult testicular SGC707 Supplier into mouse testis is one of the major bioassays for germline potential. Using this assay together with FACS to isolate putative SSCs reveals that colony-forming potential is enriched in germ cells with

• br Conclusions and future perspectives It has been suggested

  2018-10-20

  

  Conclusions and future perspectives It has been suggested that developmental mechanisms are prisoners of their own phylogenetic histories (Goss, 1991). On the basis of recent studies of cardiac regeneration in fetal/neonatal mammals, it would appear that regenerative mechanisms are also prisoners

• Time lapse images were taken on an inverted Zeiss

  2018-10-20

  

  Time-lapse images were taken on an inverted Zeiss LSM 510 microscope using Plan-Neofluar 20× (0.8 N.A.) objective. For Ca analysis, images were taken every 1.5s for 3min using 512×512 pixel resolution and cells were excited using the 488nm laser line. On each cell soma a 5μM×5μM ROI was drawn and ch

• We also observed this inside out layering again when we

  2018-10-20

  

  We also observed this inside-out layering again when we stained for nestin and MAP2, with strong nestin staining on the cells and processes growing at the outer 75μM of the SFEB (Fig. 2C), an observation we have made with Tuj1 staining as well (Fig. 1E). Within our SFEBs, nestin expression decreased

• Introduction Bone marrow BM derived

  2018-10-20

  

  Introduction Bone marrow (BM)-derived endothelial progenitor N6022 (EPCs) circulate in peripheral blood and play an important role in the formation of new blood vessels (Asahara et al., 1997; Takahashi et al., 1999; Zhou et al., 2013; Li et al., 2011). This process occurs in response to a variety

• In demyelinating diseases injury of myelin oligodendrocytes

  2018-10-20

  

  In demyelinating diseases, injury of myelin/oligodendrocytes results in a profound loss of myelin sheaths, axonal injury and degeneration, which eventually lead to long-lasting functional disabilities (Franklin and Ffrench-Constant, 2008; Emery, 2010). Mouse models of oligodendrocyte injury, such a

• br Material methods br Results br Discussion Several

  2018-10-20

  

  Material & methods Results Discussion Several LSC culture systems have been developed to re-create the LSC niche in vitro and to avoid the cross-contamination with non-human feeder molecular weight mg (Sharma et al., 2012; Xie et al., 2012; Chen et al., 2013; Mei et al., 2014). It has been

• br The ethics of cell therapy Clearly then cell

  2018-10-20

  

  The ethics of cell therapy Clearly then, cell therapy and regenerative medicine in general raise a number of ethical, economical and regulatory issues that were addressed in an ad hoc session. As a general introduction to this very complex landscape, John Harris (University of Manchester) took le

• Our facility was not licensed

  2018-10-20

  

  Our facility was not licensed by the US FDA for the manufacture of biologics to US guidelines standards for current Good Tissue Practice. However, our interpretation of these guidelines and standards (Table 2) is that they do not differ substantially from the requirements of EC directives and of HFE

• Introduction The vasculature is a multicellular system in

  2018-10-20

  

  Introduction The vasculature is a multicellular system in which each cell type plays an important and indispensible role in its function. The inner lining of endothelial cells (ECs), which are in direct contact with the blood, is surrounded and supported by perivascular cells—either vascular smooth

• br Results We investigated the identity

  2018-10-20

  

  Results We investigated the identity of human NPCs extracted from the foreskin dermis through fluorescence-activated cell sorting (FACS)-based isolation of cell subpopulations and subsequent characterization of the sorted myd88 pathway (Figure 1). Immunofluorescent and flow-cytometry analyses of

• MPCs are decidedly different from

  2018-10-20

  

  MPCs are decidedly different from the recently described CPCs (Koelling et al., 2009; Koelling and Miosge, 2010) not only due to their tissue origin and stem cell marker pattern, but also due to their ability to produce collagen type I and type II. However, they are similar as they also appear to be

• Genetic manipulation by overexpression of exogenous factors

  2018-10-20

  

  Genetic manipulation by overexpression of exogenous factors such as Nanog, Klf2, and Prdm14 enables conversion of mouse EpiSCs to ESC-like cells (rESCs) (Gillich et al., 2012; Silva et al., 2009). Furthermore, transition of mouse EpiSCs to rESCs rarely occurs even after stimulation with LIF-STAT3 si

• br Conclusion The use of SR at

  2018-10-20

  

  Conclusion The use of SR1 at 20% O2 proved to be the best condition for the expansion of HPSCs into larger cell numbers; however, the use of SR1 at 1% O2 resulted in an increase in the proportion of primitive HSCs in the sample. It will in the future be necessary to confirm the effectiveness and

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