• Conclusions can be made from many in vitro studies including

  2018-10-22

  

  Conclusions can be made from many in vitro studies including those mentioned above that citrus flavonoids exert strong anti-cancer activities. However, the beneficial effectiveness of a drug or a nutrient can only be attested by in vivo efficacy studies. In this review, we summarize the in vivo anti

• br Key problems that need to be

  2018-10-22

  

  Key problems that need to be resolved to understand the relationship between foods and health In term of the relationship between foods and health, the following four key problems have to be addressed: (1) Undernutrition causes nutrition deficiency, and overnutrition leads to obesity, hypertensio

• The use of MaSCs from GFP mice

  2018-10-22

  

  The use of MaSCs from GFP mice for in vivo transplant into wild type recipients enabled us to examine the purity of FACS-gated basal and luminal cell populations from the regenerated GFP glands. All epithelial amyloid (GFP positive) were converged in the CD24+ population, while the combination of C

• Mouse nhpESC chimeras were also prepared using the

  2018-10-22

  

  Mouse–nhpESC chimeras were also prepared using the mouse tetraploid complementation assay (Nagy, 2003). We electrofused mouse 2-cell embryos to produce 4N embryos and permitted these to develop to the expanded blastocyst stage before injecting them with GFP-expressing nhpESCs and performing embryo t

• Microarray data was validated using quantitative PCR

  2018-10-22

  

  Microarray data was validated using quantitative PCR (qPCR). This was first performed on a set of genes chosen for their wide variation in gene KY02111 between the three cell types (Prl2c2, GHR, Zyx, Des) (Supplementary Figure S2). qPCR was then performed to validate the expression of genes regarde

• We derived ESC lines from

  2018-10-22

  

  We derived ESC lines from blastocysts of the double transgenic mouse line Mlc2v-Cre; Rosa26-Yfp, in which Cre and Yfp are specifically knocked into the Mlc2v and Rosa26 gene loci, respectively. Mlc2v-Cre-mediated removal of a stop sequence in the Rosa26-YFP reporter line results in expression of YFP

• br Introduction Free fatty acids FFAs including saturated an

  2018-10-22

  

  Introduction Free fatty acids (FFAs), including saturated and unsaturated FFAs, are the major source of lipid energy in human body and released mainly from adipose tissue by lipolysis of triglycerides (Miles and Nelson, 2007). FFAs circulating in the plasma are largely bound to the albumin, with

• We studied four independent hESC lines HES H

  2018-10-22

  

  We studied four independent hESC lines: HES3, H1, ESI-035, and Shef5. Cultures from these four cell lines that had been reported to contain a 20q11.21 CNV were paired with cultures from previous passages that did not appear to be positive for the CNV by SNP array (Amps et al., 2011). Hereafter, we r

• br Discussion Xenotransplantation of human and nonhuman prim

  2018-10-22

  

  Discussion Xenotransplantation of human and nonhuman primate adult testicular SGC707 Supplier into mouse testis is one of the major bioassays for germline potential. Using this assay together with FACS to isolate putative SSCs reveals that colony-forming potential is enriched in germ cells with

• br Conclusions and future perspectives It has been suggested

  2018-10-20

  

  Conclusions and future perspectives It has been suggested that developmental mechanisms are prisoners of their own phylogenetic histories (Goss, 1991). On the basis of recent studies of cardiac regeneration in fetal/neonatal mammals, it would appear that regenerative mechanisms are also prisoners

• Time lapse images were taken on an inverted Zeiss

  2018-10-20

  

  Time-lapse images were taken on an inverted Zeiss LSM 510 microscope using Plan-Neofluar 20× (0.8 N.A.) objective. For Ca analysis, images were taken every 1.5s for 3min using 512×512 pixel resolution and cells were excited using the 488nm laser line. On each cell soma a 5μM×5μM ROI was drawn and ch

• We also observed this inside out layering again when we

  2018-10-20

  

  We also observed this inside-out layering again when we stained for nestin and MAP2, with strong nestin staining on the cells and processes growing at the outer 75μM of the SFEB (Fig. 2C), an observation we have made with Tuj1 staining as well (Fig. 1E). Within our SFEBs, nestin expression decreased

• Introduction Bone marrow BM derived

  2018-10-20

  

  Introduction Bone marrow (BM)-derived endothelial progenitor N6022 (EPCs) circulate in peripheral blood and play an important role in the formation of new blood vessels (Asahara et al., 1997; Takahashi et al., 1999; Zhou et al., 2013; Li et al., 2011). This process occurs in response to a variety

• In demyelinating diseases injury of myelin oligodendrocytes

  2018-10-20

  

  In demyelinating diseases, injury of myelin/oligodendrocytes results in a profound loss of myelin sheaths, axonal injury and degeneration, which eventually lead to long-lasting functional disabilities (Franklin and Ffrench-Constant, 2008; Emery, 2010). Mouse models of oligodendrocyte injury, such a

• br Material methods br Results br Discussion Several

  2018-10-20

  

  Material & methods Results Discussion Several LSC culture systems have been developed to re-create the LSC niche in vitro and to avoid the cross-contamination with non-human feeder molecular weight mg (Sharma et al., 2012; Xie et al., 2012; Chen et al., 2013; Mei et al., 2014). It has been

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