br Material methods br Results br Discussion Several

2018-10-20

Material & methods Results Discussion Several LSC culture systems have been developed to re-create the LSC niche in vitro and to avoid the cross-contamination with non-human feeder molecular weight mg (Sharma et al., 2012; Xie et al., 2012; Chen et al., 2013; Mei et al., 2014). It has been

br The ethics of cell therapy Clearly then cell

2018-10-20

The ethics of cell therapy Clearly then, cell therapy and regenerative medicine in general raise a number of ethical, economical and regulatory issues that were addressed in an ad hoc session. As a general introduction to this very complex landscape, John Harris (University of Manchester) took le

Our facility was not licensed

2018-10-20

Our facility was not licensed by the US FDA for the manufacture of biologics to US guidelines standards for current Good Tissue Practice. However, our interpretation of these guidelines and standards (Table 2) is that they do not differ substantially from the requirements of EC directives and of HFE

Introduction The vasculature is a multicellular system in

2018-10-20

Introduction The vasculature is a multicellular system in which each cell type plays an important and indispensible role in its function. The inner lining of endothelial cells (ECs), which are in direct contact with the blood, is surrounded and supported by perivascular cells—either vascular smooth

br Results We investigated the identity

2018-10-20

Results We investigated the identity of human NPCs extracted from the foreskin dermis through fluorescence-activated cell sorting (FACS)-based isolation of cell subpopulations and subsequent characterization of the sorted myd88 pathway (Figure 1). Immunofluorescent and flow-cytometry analyses of

MPCs are decidedly different from

2018-10-20

MPCs are decidedly different from the recently described CPCs (Koelling et al., 2009; Koelling and Miosge, 2010) not only due to their tissue origin and stem cell marker pattern, but also due to their ability to produce collagen type I and type II. However, they are similar as they also appear to be

Genetic manipulation by overexpression of exogenous factors

2018-10-20

Genetic manipulation by overexpression of exogenous factors such as Nanog, Klf2, and Prdm14 enables conversion of mouse EpiSCs to ESC-like cells (rESCs) (Gillich et al., 2012; Silva et al., 2009). Furthermore, transition of mouse EpiSCs to rESCs rarely occurs even after stimulation with LIF-STAT3 si

br Conclusion The use of SR at

2018-10-20

Conclusion The use of SR1 at 20% O2 proved to be the best condition for the expansion of HPSCs into larger cell numbers; however, the use of SR1 at 1% O2 resulted in an increase in the proportion of primitive HSCs in the sample. It will in the future be necessary to confirm the effectiveness and

br Materials and methods br Results br

2018-10-20

Materials and methods Results Discussion Several in vivo and in vitro studies indicate that OPCs have substantially different properties according to their chronological age. For example, human fetal OPCs from different gestational stages exhibit differences in the myelination profile (Cui

In a recent study Scheffer

2018-10-20

In a recent study (Scheffer et al., 2015), the expression of Lmod3 during natural mouse development was found to be restricted to cochlear HCs and that of Dnah5 to vestibular HCs, while double-positive cells were not produced. In the present study, immunocytochemical analyses showed that the cochlea

AR-42 HDAC br Results br Discussion The self renewal

2018-10-20

Results Discussion The self-renewal capacity of erythroid progenitors is an important component of current protocols to generate cultured RBCs for potential therapeutic purposes. However, a major obstacle in generating the 2.5 × 1012 RBCs that constitutes a single unit of blood is the limited

At a global level the analysis of

2018-10-20

At a global level, the analysis of DNA-methylation profiles provides important insights into molecular and cell growth characteristics of PSC that would otherwise be difficult to identify. Reprogramming-associated variations in X-linked CpG methylation is of particular interest because of the comple

Hello world!

2018-07-29